Iron is a critical element in photosynthetic tissues: it is an essential constituent of the photosynthetic complexes involved in electron transport but it is toxic when it is in a free, not complexed form, because it acts as a catalyst in the production of hydroxyl radicals during the Haber-Weiss reaction. The ability of the leaves to store iron in the chloroplasts in a soluble, readily available and non toxic form is provided by ferritins, a class of proteins present in plants as well as in bacteria, fungi and animals. Goal of our research is to better understand ferritin involvement in the plant protection against various oxidative stresses, like photoinhibition or iron excess. For that purpose we isolated, from the KO Wisconsin mutants collection, an Arabidopsis mutant bearing a T-DNA insertion in the ferritin Atfer1 promoter, 40 bp upstream the starting ATG. A description of the molecular steps through which the isolation of the mutant was performed and a preliminary physiological characterization will be given.

ISOLATION OF AN ARABIDOPSIS THALIANA MUTANT KNOCKED-OUT IN THE FERRITIN (ATFER1) GENE / I. Murgia, M. Fustinoni, C. Soave. ((Intervento presentato al convegno 40° Congresso della società Italiana di Fisiologia Vegetale (SIFV) tenutosi a AbanoTerme, Italia nel 2001.

ISOLATION OF AN ARABIDOPSIS THALIANA MUTANT KNOCKED-OUT IN THE FERRITIN (ATFER1) GENE

I. Murgia
Primo
;
C. Soave
Ultimo
2001

Abstract

Iron is a critical element in photosynthetic tissues: it is an essential constituent of the photosynthetic complexes involved in electron transport but it is toxic when it is in a free, not complexed form, because it acts as a catalyst in the production of hydroxyl radicals during the Haber-Weiss reaction. The ability of the leaves to store iron in the chloroplasts in a soluble, readily available and non toxic form is provided by ferritins, a class of proteins present in plants as well as in bacteria, fungi and animals. Goal of our research is to better understand ferritin involvement in the plant protection against various oxidative stresses, like photoinhibition or iron excess. For that purpose we isolated, from the KO Wisconsin mutants collection, an Arabidopsis mutant bearing a T-DNA insertion in the ferritin Atfer1 promoter, 40 bp upstream the starting ATG. A description of the molecular steps through which the isolation of the mutant was performed and a preliminary physiological characterization will be given.
set-2001
Settore BIO/04 - Fisiologia Vegetale
Settore AGR/13 - Chimica Agraria
Settore BIO/11 - Biologia Molecolare
ISOLATION OF AN ARABIDOPSIS THALIANA MUTANT KNOCKED-OUT IN THE FERRITIN (ATFER1) GENE / I. Murgia, M. Fustinoni, C. Soave. ((Intervento presentato al convegno 40° Congresso della società Italiana di Fisiologia Vegetale (SIFV) tenutosi a AbanoTerme, Italia nel 2001.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/211715
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