The relationship between the binding of dicyclohexylcarbodiimide (DCCD) to isolated light-harvesting proteins of photosystem LT and the inhibition of chlorophyll fluorescence quenching by DCCD have been investigated. For a range of different complexes an approximately linear relationship was obtained between the efficiency of DCCD binding and the DCCD-dependent reversal of fluorescence quenching. The most efficient labeling was found for the minor light-harvesting complexes, CP29 and CP26, In the case of the former, five different preparations were compared including two reconstituted complexes in which a putative DCCD-binding site had been mutagenized. Again, an approximately linear relationship between DCCD binding and the extent of reversal of fluorescence quenching was found. However, the binding of DCCD was found to occur at least an order of magnitude faster than the change In fluorescence. The results are discussed in terms of the multiplicity of DCCD-binding sites and the influence of protein structure on both the binding of DCCD and the fluorescence quenching mechanism.

The relationship between the binding of dicyclohexylcarbodiimide and quenching of chlorophyll fluorescence in the light-harvesting proteins of photosystem II / A.V. Ruban, P. Pesaresi, U. Wacker, K.D.J. Irrgang, R. Bassi, P. Horton. - In: BIOCHEMISTRY. - ISSN 0006-2960. - 37:33(1998 Aug 18), pp. 11586-11591.

The relationship between the binding of dicyclohexylcarbodiimide and quenching of chlorophyll fluorescence in the light-harvesting proteins of photosystem II

P. Pesaresi;
1998

Abstract

The relationship between the binding of dicyclohexylcarbodiimide (DCCD) to isolated light-harvesting proteins of photosystem LT and the inhibition of chlorophyll fluorescence quenching by DCCD have been investigated. For a range of different complexes an approximately linear relationship was obtained between the efficiency of DCCD binding and the DCCD-dependent reversal of fluorescence quenching. The most efficient labeling was found for the minor light-harvesting complexes, CP29 and CP26, In the case of the former, five different preparations were compared including two reconstituted complexes in which a putative DCCD-binding site had been mutagenized. Again, an approximately linear relationship between DCCD binding and the extent of reversal of fluorescence quenching was found. However, the binding of DCCD was found to occur at least an order of magnitude faster than the change In fluorescence. The results are discussed in terms of the multiplicity of DCCD-binding sites and the influence of protein structure on both the binding of DCCD and the fluorescence quenching mechanism.
PROTON-PUMPING ACTIVITY ; ENERGY-DISSIPATION ; GREEN PLANTS ; EXCITATION-ENERGY ; MOLECULAR MECHANISM ; THYLAKOID MEMBRANES ; ALPHA-FLUORESCENCE ; CIRCULAR-DICHROISM ; REVERSIBLE CHANGES ; ESCHERICHIA-COLI
Settore BIO/10 - Biochimica
18-ago-1998
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/211623
Citazioni
  • ???jsp.display-item.citation.pmc??? 8
  • Scopus 31
  • ???jsp.display-item.citation.isi??? 33
social impact