An actin-related protein (ACLA) has been identified in the cellular slime mould Dictyostelium discoideum. The complete cDNA sequence indicates that within the actin superfamily it belongs to the ARP3 family of actin-related proteins together with Arp66B from Drosophila melanogaster, Actin2 from Bos taurus, act2 from Schizosaccharomyces pombe and possibly act2 from Caenorhabditis elegans. The ACLA mRNA is regulated during development, showing a maximum between 2 and 4 h after starvation. The protein has been expressed in E. coli and antibodies raised against it. Immunofluorescence microscopy shows that ACLA protein co-localises with mitochondria; the protein co-purifies with Dictyostelium mitochondria.

An actin-related protein from Dictyostelium discoideum is developmentally regulated and associated with mitochondria / I. Murgia, S. K. Maciver, P. Morandini. - In: FEBS LETTERS. - ISSN 0014-5793. - 360:3(1995), pp. 235-241.

An actin-related protein from Dictyostelium discoideum is developmentally regulated and associated with mitochondria

I. Murgia
Primo
;
P. Morandini
Ultimo
1995

Abstract

An actin-related protein (ACLA) has been identified in the cellular slime mould Dictyostelium discoideum. The complete cDNA sequence indicates that within the actin superfamily it belongs to the ARP3 family of actin-related proteins together with Arp66B from Drosophila melanogaster, Actin2 from Bos taurus, act2 from Schizosaccharomyces pombe and possibly act2 from Caenorhabditis elegans. The ACLA mRNA is regulated during development, showing a maximum between 2 and 4 h after starvation. The protein has been expressed in E. coli and antibodies raised against it. Immunofluorescence microscopy shows that ACLA protein co-localises with mitochondria; the protein co-purifies with Dictyostelium mitochondria.
Actin-like protein; ARP3; cDNA sequence; Immunofluorescence; Mitochondrion; Northern blot analysis
Settore BIO/04 - Fisiologia Vegetale
Settore BIO/11 - Biologia Molecolare
Settore BIO/01 - Botanica Generale
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/211283
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