Under conditions of reduced iron availability, most frequent in calcareous soils, plants induce the "Fe Deficiency Response" to improve root Fe uptake. The transcription factor FIT is essential for such a response in strategy I plants, such as Arabidopsis thaliana. From microarray analysis of Arabidopsis roots, it is known that three different cytochrome P450 genes, CYP82C4, CYP82C3 and CYP71B5 are up-regulated under Fe deficiency through a FIT-dependent pathway. We show that, out of these three P450 genes, only CYP82C4 strongly correlates with genes involved in metal uptake/transport. The CYP82C4 promoter, unlike those of CYP82C3 and CYP71B5, contains several IDE1-like sequences (iron deficiency-responsive element) as well as an RY element. While confirming that the CYP82C4 transcript accumulates in Fe-deficient Arabidopsis seedlings, with circadian fluctuations in a light-dependent way, we also demonstrate that such accumulation is suppressed under Fe excess. Full suppression of CYP82C4 expression, as observed in the atc82c4-1 KO mutant, is associated with longer roots at the seedling stage. We propose that CYP82C4 is involved in the early Fe deficiency response, possibly through an IDE1-like mediated pathway.
Arabidopsis CYP82C4 expression is dependent on Fe availability and circadian rhythm, and correlates with genes involved in the early Fe deficiency response / I. Murgia, D. Tarantino, C. Soave, P. Morandini. - In: JOURNAL OF PLANT PHYSIOLOGY. - ISSN 0176-1617. - 168:9(2011), pp. 894-902.
|Titolo:||Arabidopsis CYP82C4 expression is dependent on Fe availability and circadian rhythm, and correlates with genes involved in the early Fe deficiency response|
MURGIA, IRENE (Primo)
TARANTINO, DELIA (Secondo)
SOAVE, CARLO (Penultimo)
MORANDINI, PIERO ANGELO (Ultimo)
|Parole Chiave:||Promoter Regions, Genetic ; Plant Roots ; Arabidopsis Proteins ; Circadian Rhythm ; Cytochrome P-450 Enzyme System ; Seedling ; Arabidopsis ; Gene Expression Regulation, Plant ; Mutation ; Iron ; Mutagenesis, Insertional|
|Settore Scientifico Disciplinare:||Settore BIO/04 - Fisiologia Vegetale|
Settore AGR/13 - Chimica Agraria
|Data di pubblicazione:||2011|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.jplph.2010.11.020|
|Appare nelle tipologie:||01 - Articolo su periodico|