A mutagenised population of seeds of Arabidopsis thaliana was allowed to germinate in the presence of the positively charged aminoglycoside hygromycin (4 mu g/ml) and the fungal toxin fusicoccin (5x10(-6) M). This hygromycin concentration, which is nontoxic by itself, becomes toxic when used together with fusicoccin, which stimulates cation uptake. Seeds that had germinated after 3-5 days and produced seedlings with green cotyledons were potentially resistant to fusicoccin and were therefore transferred into sterile Magenta vessels containing 1/2-strength Murashige and Skoog medium. This selection procedure is non-destructive, i.e. it allows the recovery of viable seedlings and their growth into adult plants thus permitting direct physiological characterisation.

A non-destructive selection method for resistance to fusicoccin in Arabidopsis thaliana / I. Murgia, P. Morandini, A. Moroni, C. Soave. - In: PLANT CELL REPORTS. - ISSN 0721-7714. - 18:3-4(1998), pp. 255-259.

A non-destructive selection method for resistance to fusicoccin in Arabidopsis thaliana

I. Murgia
Primo
;
P. Morandini
Secondo
;
A. Moroni
Penultimo
;
C. Soave
Ultimo
1998

Abstract

A mutagenised population of seeds of Arabidopsis thaliana was allowed to germinate in the presence of the positively charged aminoglycoside hygromycin (4 mu g/ml) and the fungal toxin fusicoccin (5x10(-6) M). This hygromycin concentration, which is nontoxic by itself, becomes toxic when used together with fusicoccin, which stimulates cation uptake. Seeds that had germinated after 3-5 days and produced seedlings with green cotyledons were potentially resistant to fusicoccin and were therefore transferred into sterile Magenta vessels containing 1/2-strength Murashige and Skoog medium. This selection procedure is non-destructive, i.e. it allows the recovery of viable seedlings and their growth into adult plants thus permitting direct physiological characterisation.
Arabidopsis thaliana; Fusicoccin; Selection method
Settore BIO/04 - Fisiologia Vegetale
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/211256
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