A mutagenised population of seeds of Arabidopsis thaliana was allowed to germinate in the presence of the positively charged aminoglycoside hygromycin (4 mu g/ml) and the fungal toxin fusicoccin (5x10(-6) M). This hygromycin concentration, which is nontoxic by itself, becomes toxic when used together with fusicoccin, which stimulates cation uptake. Seeds that had germinated after 3-5 days and produced seedlings with green cotyledons were potentially resistant to fusicoccin and were therefore transferred into sterile Magenta vessels containing 1/2-strength Murashige and Skoog medium. This selection procedure is non-destructive, i.e. it allows the recovery of viable seedlings and their growth into adult plants thus permitting direct physiological characterisation.
A non-destructive selection method for resistance to fusicoccin in Arabidopsis thaliana / I. Murgia, P. Morandini, A. Moroni, C. Soave. - In: PLANT CELL REPORTS. - ISSN 0721-7714. - 18:3-4(1998), pp. 255-259.
|Titolo:||A non-destructive selection method for resistance to fusicoccin in Arabidopsis thaliana|
MURGIA, IRENE (Primo)
MORANDINI, PIERO ANGELO (Secondo)
MORONI, ANNA (Penultimo)
SOAVE, CARLO (Ultimo)
|Parole Chiave:||Arabidopsis thaliana; Fusicoccin; Selection method|
|Settore Scientifico Disciplinare:||Settore BIO/04 - Fisiologia Vegetale|
|Data di pubblicazione:||1998|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1007/s002990050567|
|Appare nelle tipologie:||01 - Articolo su periodico|