Media conditioned with viable rat and calf spleen lymphocytes were seen to be able to inhibit 3H-thymidine uptake by rat thymocytes and the mitogenic response of human lymphocytes to PHA but not bone marrow cells proliferation. The inhibitor appeared to have a molecular weight greater than 50,000 daltons, as determined by Amicon Diaflo ultrafiltration. Media conditioned with rat granulocytes had a similar tissue specific but species non-specific inhibitory action. Also human leukemic cells appeared able to produce in vitro an inhibitor of cell proliferation when a certain degree of differentiation was present.
In vitro production of lymphocyte and granulocyte proliferation inhibitors (chalones?) from living cells / A.T. Maiolo, E. Cazzaniga, A. Cortelezzi, V. De Pangher, P. Foa, L. Lombardi, R. Mozzana, E.E. Polli. - In: BOLLETTINO DELL'ISTITUTO SIEROTERAPICO MILANESE. - ISSN 0021-2547. - 54:3(1975 Jul), pp. 235-243.
In vitro production of lymphocyte and granulocyte proliferation inhibitors (chalones?) from living cells
A.T. MaioloPrimo
;A. Cortelezzi;P. Foa;
1975
Abstract
Media conditioned with viable rat and calf spleen lymphocytes were seen to be able to inhibit 3H-thymidine uptake by rat thymocytes and the mitogenic response of human lymphocytes to PHA but not bone marrow cells proliferation. The inhibitor appeared to have a molecular weight greater than 50,000 daltons, as determined by Amicon Diaflo ultrafiltration. Media conditioned with rat granulocytes had a similar tissue specific but species non-specific inhibitory action. Also human leukemic cells appeared able to produce in vitro an inhibitor of cell proliferation when a certain degree of differentiation was present.
Pubblicazioni consigliate
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
