A novel approach of competitive PCR for quantifying mRNAs is described where competitors are constructed by deleting an internal sequence from target reverse transcribed mRNAs. The two sets of product generated (control,and target) are easily discriminated by size and quantified for the sequence of interest and a housekeeping gene, beta-actin used as internal mRNA control.
Rapid construction of competitive templates for quantitative PCR of reverse-transcribed mRNAs / L. Del Giacco, L. Doneda, P. Grammatico, L. Larizza. - In: BIOTECHNOLOGY TECHNIQUES. - ISSN 0951-208X. - 9:12(1995), pp. 879-884.
Rapid construction of competitive templates for quantitative PCR of reverse-transcribed mRNAs
L. Del GiaccoPrimo
;L. DonedaSecondo
;L. LarizzaUltimo
1995
Abstract
A novel approach of competitive PCR for quantifying mRNAs is described where competitors are constructed by deleting an internal sequence from target reverse transcribed mRNAs. The two sets of product generated (control,and target) are easily discriminated by size and quantified for the sequence of interest and a housekeeping gene, beta-actin used as internal mRNA control.
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