Zebrafish represents an excellent model to study the function of vertebrate genes (e.g., well-developed genetics, large number of mutants, and genomic sequencing in progress), inasmuch as we have tools to manipulate gene expression. Recent use of injected morpholinos in eggs provides a good method to " knockdown " gene expression in early development (Nasevicius and Ekker, 2000), and the "caged" RNA injected in eggs allows to overexpress a gene in a specific set of cells (Ando et al., 2001). However, a method to specifically modify gene expression in the juvenile or in the adult is still missing. Such a method would be a very powerful tool to understand gene function in differentiated tissues. We describe here an electroporation-based approach, which allows gene transfer in adult tissues. Its efficiency was assessed using a GFP (green fluorescent protein) dependent assay. We then used this method to disrupt the Fgf signalling pathway during the process of regeneration.
|Titolo:||High-Efficiency Gene Transfer into Adult Fish : a New Tool to Study Fin Regeneration|
|Parole Chiave:||Dominant-negative mutation; Electroporation; Fgf; Fgf receptor 1; Fin; Regeneration; Zebrafish|
|Settore Scientifico Disciplinare:||Settore MED/26 - Neurologia|
|Data di pubblicazione:||gen-2002|
|Digital Object Identifier (DOI):||10.1002/gene.10025|
|Appare nelle tipologie:||01 - Articolo su periodico|