We are performing a longitudinal survey to investigate AIDS related leukoencephalopathies, including PML and JC virus (JCV) negative leukoencephalopathy (NDLE). HIV+ HAART treated patients are subjected to MRI examination, CSF was studied for the presence of JCV and all the human herpesviruses. HIV and JCV viral loads and MCP-1 were measured in CSF. PBMC production of TNFa, IFNg, IL-2 and MCP-1, previous and after JCV HLA-restricted peptides stimulation was performed by flow cytometry. 22 MRI+ (8 PML,12 NDLE , 2 OND),41 MRI- (30 asymptomatic, 11 OND) were studied with 27 healthy controls. Plasma, but not CSF, HIV load was higher (p<0.05) in PML than in NDLE . MCP-1 and HIV RNA in CSF were positively correlated in the three groups (PML: r=0.11 p=0.89; NDLE r: 0.39 p:0.19; OND: r:0.26 p: 0.39). No viruses were found in NDLE CSF, whereas VZV and EBV were found in CSF of 2 OND MRI+ . IFNg was overproduced (p<0.05) in CD4+ cells of NDLE and PML. The JCV specific immune response and increased cytotoxic activity suggests that JCV, with an immune mediated mechanism, is relevant in NDLE pathogenesis
VIRUS-SPECIFIC IMMUNE RESPONSE IMBALANCE IN HIV ASSOCIATED LEUKOENCEPHALOPATHIES / P. Ferrante, S. Delbue, M. Saresella, E. Colombo, F.R. Guerini, M. Valli, I. Marventano, R. Mancuso, G. Sotgiu, R. Maserati. ((Intervento presentato al 11. convegno Conference of society on neuroimmune pharmacology on neuroimmune circuits and infectious diseases tenutosi a Clearwater beach nel 2005.
VIRUS-SPECIFIC IMMUNE RESPONSE IMBALANCE IN HIV ASSOCIATED LEUKOENCEPHALOPATHIES
S. Delbue;
2005
Abstract
We are performing a longitudinal survey to investigate AIDS related leukoencephalopathies, including PML and JC virus (JCV) negative leukoencephalopathy (NDLE). HIV+ HAART treated patients are subjected to MRI examination, CSF was studied for the presence of JCV and all the human herpesviruses. HIV and JCV viral loads and MCP-1 were measured in CSF. PBMC production of TNFa, IFNg, IL-2 and MCP-1, previous and after JCV HLA-restricted peptides stimulation was performed by flow cytometry. 22 MRI+ (8 PML,12 NDLE , 2 OND),41 MRI- (30 asymptomatic, 11 OND) were studied with 27 healthy controls. Plasma, but not CSF, HIV load was higher (p<0.05) in PML than in NDLE . MCP-1 and HIV RNA in CSF were positively correlated in the three groups (PML: r=0.11 p=0.89; NDLE r: 0.39 p:0.19; OND: r:0.26 p: 0.39). No viruses were found in NDLE CSF, whereas VZV and EBV were found in CSF of 2 OND MRI+ . IFNg was overproduced (p<0.05) in CD4+ cells of NDLE and PML. The JCV specific immune response and increased cytotoxic activity suggests that JCV, with an immune mediated mechanism, is relevant in NDLE pathogenesis
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