27-Hydroxycholesterol**, 25-hydroxycholesterol and cholesterol suppressed LDL uptake and degradation in human extrahepatic and hepatic cell lines in a concentration-dependent manner. Cholesterol was the least potent, and the inhibitory effect of oxysterols was more pronounced in skin fibroblasts and in endothelial cell line EAhy 926 than in hepatoma HepG2 cells. Shorter incubations were required for oxysterols to achieve 50% inhibition of LDL uptake and degradation in all cultured cells. The inhibition of LDL catabolism in extrahepatic cells by 27-hydroxycholesterol occurred at concentrations close to those observed in human plasma (0.2-0.6 microM). The results support a possible role of 27-hydroxycholesterol, a physiological oxysterol, in the regulation of cellular cholesterol homeostasis in non-hepatic tissues.
27-Hydroxycholesterol modulation of low density lipoprotein metabolism in cultured human hepatic and extrahepatic cells / S. Bellosta, A. Corsini, F. Bernini, A. Granata, G. Didoni, M. Mazzotti, R. Fumagalli. - In: FEBS LETTERS. - ISSN 0014-5793. - 332:1-2(1993 Oct 11), pp. 115-118.
|Titolo:||27-Hydroxycholesterol modulation of low density lipoprotein metabolism in cultured human hepatic and extrahepatic cells|
BELLOSTA, STEFANO (Primo)
CORSINI, ALBERTO (Secondo)
|Parole Chiave:||Human endothelial cell; Human fibroblast; Human hepatoma cell line Hep G2; LDL receptor; Oxysterol|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||11-ott-1993|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/0014-5793(93)80495-G|
|Appare nelle tipologie:||01 - Articolo su periodico|