Background: Allergy to Prunoideae fruit (plum, peach, cherry and apricot) is one of the most frequent food allergies in southern Europe. All these fruits cross-react in vivo and in vitro, as they share their major allergen, a 9 kD lipid transfer protein (LTP). Objective: The aim of the study was the identification and molecular characterization of the major allergen of plum. Methods: The IgE pattern of reactivity to plums was investigated by SDS-PAGE and immunoblotting with the sera of 23 patients. The identified major allergen was purified by HPLC, using a cationic-exchange column followed by gel-filtration. Further characterization was achieved by periodic-Schiff stain, isoelectrofocusing and N-terminal amino acid sequencing. Results and conclusions: The major allergen of plum is a 9 kD lipid transfer protein, not glycosylated and with a basic character (pI > 9), highly homologous to the major allergen of peach.
|Titolo:||Characterization of the major allergen of plum as a lipid transfer protein|
PASTORELLO, ELIDE ANNA (Primo)
|Parole Chiave:||food allergy ; skin prick test ; Prunoideae fruit ; lipid transfer proteins|
|Settore Scientifico Disciplinare:||Settore MED/09 - Medicina Interna|
|Data di pubblicazione:||mag-2001|
|Digital Object Identifier (DOI):||10.1016/S0378-4347(01)00074-3|
|Appare nelle tipologie:||01 - Articolo su periodico|