The complete amino acid sequence of a basic (pI 9.0) fatty acid-binding protein purified from liver of Gallus domesticus was determined by automated Edman degradation of tryptic, CNBr/HFBA and Staphylococcus aureus protease peptides. The protein contains 125 amino acid residues which correspond to a molecular mass of 14094. The identification of the blocked N-terminus Ac-Ala required digestion of a SV-8 peptide with the acylamino acid-releasing enzyme prior to sequence analysis. Sequence comparison shows that chicken liver basic-FABP has a significant similarity to other proteins belonging to the superfamily of intracellular lipid molecule binding proteins. Moreover, these sequence data confirm that basic-FABP probably binds its substrate in a slightly different way when compared with other FABPs. Basic-FABP was submitted to the EMBL Data Library with an accession number of P80226.
|Titolo:||The primary structure of a basic (pI-9.0) fatty-acid-binding protein from liver of Gallus domesticus|
CECILIANI, FABRIZIO (Primo)
|Parole Chiave:||Animals ; Fatty Acid-Binding Proteins ; Chickens ; Neoplasm Proteins ; Carrier Proteins ; Molecular Sequence Data ; Liver ; Amino Acid Sequence ; Alkalies ; Myelin P2 Protein|
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||1994|
|Digital Object Identifier (DOI):||10.1016/0305-0491(94)90010-8|
|Appare nelle tipologie:||01 - Articolo su periodico|