The major protein allergen of peach (Prunus persica), Pru p1, has recently been identified as a lipid transfer protein (LTP), The complete primary structure of Pru p1, obtained by direct amino acid sequence and liquid chromatography-mass spectrometry (LC-MS) analyses with the purified protein, is described here. The protein consists of 91 amino acids with a calculated molecular mass of 9178 Da. The amino acid sequence contains eight strictly conserved cysteines, as do all known LTPs, but secondary structure predictions failed to classify the peach 9 kDa protein as an 'all-alpha type', due to the high frequency of amino acids (nine prolines) disrupting alpha helices, Although the sequence similarity with maize LTP is only 63%, out of the 25 amino acids forming the inner surface of the tunnel-like hydrophobic cavity in maize ns-LTP 16 are identical and 7 similar in the peach homolog, supporting the hypothesis of a similar function.
|Titolo:||Complete amino acid sequence determination of the major allergen of peach (Prunus persica) Pru p 1|
PASTORELLO, ELIDE ANNA (Primo)
|Parole Chiave:||lipid transfer protein ; primary structure ; rosaceae|
|Settore Scientifico Disciplinare:||Settore MED/09 - Medicina Interna|
|Data di pubblicazione:||1999|
|Digital Object Identifier (DOI):||10.1515/BC.1999.167|
|Appare nelle tipologie:||01 - Articolo su periodico|