Background: Only a few food allergens have as yet been identified, mainly because of the difficulty of obtaining a sufficient number of patients who are clinically sensitized to a given food. This is more feasible in the case of the oral allergy syndrome (OAS), a common form of food allergy, which is especially prevalent in patients with pollinosis. Objective: We designed a study to identify the allergens of kiwi fruit (Actinidia chinensis) by analyzing the sera of patients with OAS for kiwi and to examine the cross-reactivity of these allergens with timothy and birch pollen. allergens. Methods: Twenty-seven patients with OAS for kiwi, a positive skin prick test response and serum IgE antibody to kiwi, and a positive open kiwi challenge test result and three patients who had OAS with severe systemic symptoms, which excluded a challenge test, were included in this study. The different polypeptide components of an extinct of fresh kiwi were separated by sodium dodecylsulfate-polyacrylamide gel electrophoresis and analyzed by IgE immunoblotting with sera from these patients. Cross-reactivity with the two pollen. extracts was assessed by inhibition of the immunoblots with pooled and individual patients' sera. Results: Twelve IgE-binding components with molecular weights ranging from 12 to 64 kd were identified in the kiwi extract, bur only a 30 kd component acted as major allergen, being recognized by sera of 100% of these patients. Inhibition of kiwi immunoblots with timothy and birch pollen extracts demonstrated strong cross-reactivity with some of the kiwi allergens, suggesting complete identity between certain food and pollen allergens; whereas others, particularly the 30 kd allergen were only partially inhibited suggesting much weaker cross-reactivity. Conclusions: Kiwi fruit contains a large number of allergens widely cross-reacting with allergens in grass and birch pollen extracts. Nevertheless, the major allergen at 30 kd appears to be specific for kiwi.
|Titolo:||Identification of the allergenic components of kiwi fruit and evaluation of their cross-reactivity with timothy and birch pollens|
PASTORELLO, ELIDE ANNA (Primo)
|Parole Chiave:||kiwi ; oral allergy syndrome ; food allergy ; cross-allergenicity ; immunoblotting ; timothy ; birch|
|Settore Scientifico Disciplinare:||Settore MED/09 - Medicina Interna|
|Data di pubblicazione:||set-1996|
|Digital Object Identifier (DOI):||10.1016/S0091-6749(96)70094-5|
|Appare nelle tipologie:||01 - Articolo su periodico|