The identification of food allergens is a priority in the management of food allergy, because of the need to obtain standardized extracts and pure allergens for diagnosis and therapy. It is thus important to develop methods for purification of allergenic molecules in order to study their biological and immunological characteristics. Protocols for protein extraction from foods and for allergen purification are reviewed in this paper. We report published methods for extraction of allergens from either animal and vegetable foods and detailed purification methodologies including ion-exchange, gel filtration and reversed-phase chromatography of well known allergens.
|Titolo:||Isolation of food allergens|
PASTORELLO, ELIDE ANNA (Primo)
|Parole Chiave:||reviews ; food allergy ; skin prick test ; radioallergosorbent assay|
|Settore Scientifico Disciplinare:||Settore MED/09 - Medicina Interna|
|Data di pubblicazione:||mag-2001|
|Digital Object Identifier (DOI):||10.1016/S0378-4347(01)00072-X|
|Appare nelle tipologie:||01 - Articolo su periodico|