OBJECTIVES: although the mechanism of arterial dilation and aneurysm development has not been clarified, the degradation of elastin and collagen plays undoubtedly a critical role. We evaluated the elastin and collagen content through the detection of their cross-links in aneurysmal and non-aneurysmal abdominal aortic walls. MATERIALS AND METHODS: in 26 human abdominal aortic aneurysm specimens obtained during surgery and in 24 autopsy control samples of non-aneurysmal abdominal aorta the tissue content of elastin and collagen cross-links were measured by HPLC. Collagen was also detected by evaluating two characteristic amino acids, 4-hydroxyproline (4-hypro) with a colorimetric method and 5-hydroxylysine (5-hylys) by gas chromatography. RESULTS: significantly fewer elastin cross-links were found in aneurysm samples compared to controls (desmosines and isodesmosines: 90% reduction; p<0.01). The opposite was true for pyridinoline collagen cross-links (350% increase) and deoxypyridinolines (100% increase, p=0.01). Tissue content of 5-hylys, 4-hypro and total amino acids were reduced significantly by 50% in aneurysmal samples. CONCLUSIONS: beside confirming decreased elastin content in aneurysmal walls, these results show a concurrent increase of collagen cross-links. Since total collagen markers were decreased (decreased 4-hypro and 5-hylys) it is reasonable to suggest that in aneurysmal aortic walls old collagen accumulates cross-links while new collagen biosynthesis is somehow defective.
Alteration of elastin, collagen and their cross-links in abdominal aortic aneurysms / M. Carmo, L. Colombo, A. Bruno, F.R.M. Corsi, L. Roncoroni, M.S. Cuttin, F. Radice, E. Mussini, P.G. Settembrini. - In: EUROPEAN JOURNAL OF VASCULAR AND ENDOVASCULAR SURGERY. - ISSN 1078-5884. - 23:6(2002 Jun), pp. 543-549.
|Titolo:||Alteration of elastin, collagen and their cross-links in abdominal aortic aneurysms|
|Parole Chiave:||Abdominal aortic aneurysm/etiology; Collagen; Cross-links; Elastin; HPLC|
|Settore Scientifico Disciplinare:||Settore MED/22 - Chirurgia Vascolare|
Settore MED/18 - Chirurgia Generale
Settore BIO/14 - Farmacologia
|Data di pubblicazione:||giu-2002|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1053/ejvs.2002.1620|
|Appare nelle tipologie:||01 - Articolo su periodico|