Recently published reports have described possible cellular therapy approaches to regenerate muscle tissues using arterial route delivery. However, the kinetic of distribution of these migratory stem cells within injected animal muscular dystrophy models is unknown. Using living X-ray computed microtomography, we established that intra-arterially injected stem cells traffic to multiple muscle tissues for several hours until their migration within dystrophic muscles. Injected stem cells express multiple traffic molecules, including VLA-4, LFA-1, CD44, and the chemokine receptor CXCR4, which are likely to direct these cells into dystrophic muscles. In fact, the majority of intra-arterially injected stem cells access the muscle tissues not immediately after the injection, but after several rounds of recirculation. We set up a new, living, 3D-imaging approach, which appears to be an important way to investigate the kinetic of distribution of systemically injected stem cells within dystrophic muscle tissues, thereby providing supportive data for future clinical applications

Novel insight into stem cell trafficking in dystrophic muscles / A. Farini, C. Villa, A. Manescu, F. Fiori, A. Giuliani, P. Razini, C. Sitzia, G. Del Fraro, M. Belicchi, M. Meregalli, F. Rustichelli, Y. Torrente. - In: INTERNATIONAL JOURNAL OF NANOMEDICINE. - ISSN 1176-9114. - 2012:7(2012 May), pp. 3059-3067. [10.2147/IJN.S30595]

Novel insight into stem cell trafficking in dystrophic muscles

A. Farini
Primo
;
C. Villa;P. Razini;M. Belicchi;M. Meregalli;Y. Torrente
Ultimo
2012

Abstract

Recently published reports have described possible cellular therapy approaches to regenerate muscle tissues using arterial route delivery. However, the kinetic of distribution of these migratory stem cells within injected animal muscular dystrophy models is unknown. Using living X-ray computed microtomography, we established that intra-arterially injected stem cells traffic to multiple muscle tissues for several hours until their migration within dystrophic muscles. Injected stem cells express multiple traffic molecules, including VLA-4, LFA-1, CD44, and the chemokine receptor CXCR4, which are likely to direct these cells into dystrophic muscles. In fact, the majority of intra-arterially injected stem cells access the muscle tissues not immediately after the injection, but after several rounds of recirculation. We set up a new, living, 3D-imaging approach, which appears to be an important way to investigate the kinetic of distribution of systemically injected stem cells within dystrophic muscle tissues, thereby providing supportive data for future clinical applications
iron nanoparticles ; micro-CT ; CD133+ stem cells ; dystrophic muscles
Settore BIO/13 - Biologia Applicata
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/206630
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