We verified the diagnostic and prognostic role of a simplified immunophenotypic classification (IC) in a series of 258 patients (M/F: 1.4; median age: 64 years; median follow-up: 64 months; 75 deaths) with mature B cell lymphoid leukemias (MBC-LL) for whom no histopathological diagnosis was available because of minimal or no lymph node involvement. The IC was based on the reactivity of three pivotal immunophenotypic markers: CD5, CD23 and SIg intensity. On the basis of different expression patterns, we identified four diagnostic clusters (C) characterized by distinct clinico-biological features and different prognoses: C1 (149 patients) identified most classical B cell chronic lymphocytic leukemias (CLL-type cluster; SIg(dim)/CD5+/CD23+); C2, 38 patients whose clinico-hematological characteristics were intermediate between C1 and C3 (CLL-variant cluster; SIg(bright)/CD5+/CD23+/-or SIg(dim)/CD5-/-/CD23 indifferent); C3 (16 patients) most situations consistent with mantle cell lymphoma in leukemic phase (MCL-type cluster; SIg(bright)/CD5+/CD23-); and C4, 55 cases, most of whom were consistent with leukemic phase lymphoplasmacytic/splenic marginal zone lymphomas (LP/S-type cluster; SIg(bright)/CD5-/+/CD23 indifferent). At univariate survival analysis, prognosis worsened from C1 to C4, C2 and C3 (P = 0.0001), and this was maintained at multivariate analysis (P = 0.006), together with CD11c expression (P = 0.0043), age at diagnosis (cut-off 70 years; P = 0.0008) and platelet count (cut-off 140 x 10(9)/l; P = 0.0034). Besides recognising the two well-known situations of classic B-CLL and MCL, our IC identified situations with distinct prognostic and/or clinical behaviors.
Diagnostic role and prognostic significance of a simplified immunophenotypic classification of mature B cell chronic lymphoid leukemias / L. Cro, A. Guffanti, M. Colombi, B. Cesana, M.G. Grimoldi, C. Patriarca, M. Goldaniga, A. Neri, D. Intini, A. Cortelezzi, A.T. Maiolo, L. Baldini. - In: LEUKEMIA. - ISSN 0887-6924. - 17:1(2003 Jan), pp. 125-132.
|Titolo:||Diagnostic role and prognostic significance of a simplified immunophenotypic classification of mature B cell chronic lymphoid leukemias|
MAIOLO, ANNA TERESA (Penultimo)
BALDINI, LUCA (Ultimo)
|Parole Chiave:||sensitivity and specificity ; karyotyping ; humans ; aged ; lymphocytes ; gene expression regulation, neoplastic ; genes, bcl-1 ; transcription factors ; aged, 80 and over ; leukemia, lymphocytic, chronic, B-cell ; proto-oncogene proteins c-bcl-6 ; antigens, CD ; adult ; male ; immunoglobulins ; DNA-binding proteins ; lectins ; membrane proteins ; receptors, IgE ; blotting, Western ; proto-oncogene proteins ; survival rate ; antigens, CD5 ; chromosome aberrations ; follow-up studies ; middle aged ; immunophenotyping ; female|
|Settore Scientifico Disciplinare:||Settore MED/08 - Anatomia Patologica|
Settore MED/15 - Malattie del Sangue
|Data di pubblicazione:||gen-2003|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1038/sj.leu.2402737|
|Appare nelle tipologie:||01 - Articolo su periodico|