A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples.
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction / P. Chomczynski, N. Sacchi. - In: ANALYTICAL BIOCHEMISTRY. - ISSN 0003-2697. - 162:1(1987 Apr), pp. 156-159.
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
N. SacchiUltimo
1987
Abstract
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples.
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