Anaplastic large-cell lymphomas (ALCLs) are a group of clinically and biologically heterogeneous diseases including the ALK(+) and ALK(-) systemic forms. Whereas ALK(+) ALCLs are molecularly characterized and can be readily diagnosed, specific immunophenotypic or genetic features to define ALK(-) ALCL are missing, and their distinction from other T-cell non-Hodgkin lymphomas (T-NHLs) remains controversial. In the present study, we undertook a transcriptional profiling meta-analysis of 309 cases, including ALCL and other primary T-NHL samples. Pathway discovery and prediction analyses defined a minimum set of genes capable of recognizing ALK(-) ALCL. Application of quantitative RT-PCR in independent datasets from cryopreserved and formalin-fixed paraffin-embedded samples validated a 3-gene model (TNFRSF8, BATF3, and TMOD1) able to successfully separate ALK(-) ALCL from peripheral T-cell lymphoma not otherwise specified, with overall accuracy near 97%. In conclusion, our data justify the possibility of translating quantitative RT-PCR protocols to routine clinical settings as a new approach to objectively dissect T-NHL and to select more appropriate therapeutic protocols.
Identification of a 3-gene model as a powerful diagnostic tool for the recognition of ALK-negative anaplastic large-cell lymphoma / L. Agnelli, E. Mereu, E. Pellegrino, T. Limongi, I. Kwee, E. Bergaggio, M. Ponzoni, A. Zamò, J. Iqbal, P.P. Piccaluga, A. Neri, W.C. Chan, S. Pileri, F. Bertoni, G. Inghirami, R. Piva. - In: BLOOD. - ISSN 0006-4971. - 120:6(2012 Aug 09), pp. 1274-1281.
|Titolo:||Identification of a 3-gene model as a powerful diagnostic tool for the recognition of ALK-negative anaplastic large-cell lymphoma|
AGNELLI, LUCA (Primo)
|Settore Scientifico Disciplinare:||Settore MED/15 - Malattie del Sangue|
|Data di pubblicazione:||9-ago-2012|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1182/blood-2012-01-405555|
|Appare nelle tipologie:||01 - Articolo su periodico|