The objective of this study was to create a stable experimental model to act as a living incubator for the main important intestinal bacteria. We have therefore inoculated germ-free mice with the most important bacteria of the human intestinal microflora, in order to study the effect of some oral antibiotics on the intestinal microflora. Sixty germ-free mice, 7 weeks old and of either sex, were inoculated orally with human faecal bacteria by means of their drinking water. Administrations were made at regular intervals following a scheme that respected some of the metabolic inter-relationships of the microorganisms used. The results showed that colonization of the germ-free mouse intestines had been achieved by most of the bacteria that had been inoculated. This "coculture" was stable in time, contrary to what happens when in-toto lyophilized faeces are administered, and the bacterial concentrations for each strains were similar to those found in human faeces.
An experimental model to reproduce some bacterial intestinal cocultures in germ-free mice / M.R. Gismondo, L. Drago, A. Lombardi, C. Fassina. - In: DRUGS UNDER EXPERIMENTAL AND CLINICAL RESEARCH. - ISSN 0378-6501. - 20:4(1994), pp. 149-152.
|Titolo:||An experimental model to reproduce some bacterial intestinal cocultures in germ-free mice|
GISMONDO, MARIA RITA (Primo)
DRAGO, LORENZO (Secondo)
LOMBARDI, ANNA (Penultimo)
|Settore Scientifico Disciplinare:||Settore MED/46 - Scienze Tecniche di Medicina di Laboratorio|
|Data di pubblicazione:||1994|
|Appare nelle tipologie:||01 - Articolo su periodico|