Interspecies human x mouse cell hybrids were used to investigate the genetic basis of human permissivity to HTLV-III(B) infection. T cell hybrids between the mouse BW 51.47 T lymphoma line and normal, PHA-IL-2 activated, human peripheral mononuclear cells (PBMCs) were generated. These hybrids preferentially segregated human chromosomes, as assessed by phenotype and karyotype analysis. Viral integration occurred only in those hybrids expressing CD4+ at the cell surface. However, infectious progeny production was demonstrated only in two of the three CD4+ hybrids tested. By segregation analysis, we could correlate the absence of human chromosomes 1, 3, and 9 with the lack of infectious viral progeny.
Susceptibility of human-mouse T cell hybrids to HIV productive infection / D. Ramarli, C. Cambiaggi, C. De Giuli Morghen, P. Tripputi, R. Ortolani, M. Bolzanelli, G. Tridente, R.S. Accolla. - In: AIDS RESEARCH AND HUMAN RETROVIRUSES. - ISSN 0889-2229. - 9:12(1993), pp. 1263-1269.
Susceptibility of human-mouse T cell hybrids to HIV productive infection
C. De Giuli Morghen;P. Tripputi;
1993
Abstract
Interspecies human x mouse cell hybrids were used to investigate the genetic basis of human permissivity to HTLV-III(B) infection. T cell hybrids between the mouse BW 51.47 T lymphoma line and normal, PHA-IL-2 activated, human peripheral mononuclear cells (PBMCs) were generated. These hybrids preferentially segregated human chromosomes, as assessed by phenotype and karyotype analysis. Viral integration occurred only in those hybrids expressing CD4+ at the cell surface. However, infectious progeny production was demonstrated only in two of the three CD4+ hybrids tested. By segregation analysis, we could correlate the absence of human chromosomes 1, 3, and 9 with the lack of infectious viral progeny.
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