Three different methods for RNA preparation using guanidine are presented in this unit--a single-step isolation method employing liquid-phase separation to selectively extract total RNA from tissues and cultured cells and two methods that rely on a CsCl step gradient to isolate total RNA.
Guanidine methods for total RNA preparation / R.E. Kingston, P. Chomczynski, N. Sacchi. - In: CURRENT PROTOCOLS IN MOLECULAR BIOLOGY. - ISSN 1934-3639. - Suppl. 36:Unit 4.2(2001 May), pp. 4.2.1-4.2.9. [10.1002/0471142727.mb0402s36]
Guanidine methods for total RNA preparation
N. Sacchi
2001
Abstract
Three different methods for RNA preparation using guanidine are presented in this unit--a single-step isolation method employing liquid-phase separation to selectively extract total RNA from tissues and cultured cells and two methods that rely on a CsCl step gradient to isolate total RNA.File in questo prodotto:
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