The genomic analysis of four non-T-leukemic lines NALM-1, KM-3, NALL-1, and NALM-16 shows a rearranged configuration of the immunoglobulin mu chain genes, indicating that they represent early B stages of differentiation. The differentiative potential of these pre-B cell lines was tested using the phorbol ester tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA). Phenotypic changes in the immunological marker profile (induction of BA-1 and BA-2 antigens) are observed, presumably reflecting events occurring in precursor B cells. However, no maturation to immunoglobulin mu chain production could be obtained. The limited maturation can be explained on the basis of possible aberrant mu chain gene rearrangements, or other mechanisms of a regulatory nature, preventing the transcription and/or the translation of rearranged mu chain genes.
|Titolo:||Phorbol ester-induced changes in human leukemic pre-B lines|
SACCHI, NICOLETTA (Primo)
|Parole Chiave:||leukemia, lymphoid ; tetradecanoylphorbol acetate ; humans ; antigens, neoplasm ; cell differentiation ; neprilysin ; B-lymphocytes ; leukemia ; phorbols ; immunoglobulin mu-Chains ; stem cells ; cell line ; cell transformation, neoplastic|
|Settore Scientifico Disciplinare:||Settore BIO/11 - Biologia Molecolare|
|Data di pubblicazione:||mar-1984|
|Digital Object Identifier (DOI):||10.1016/0008-8749(84)90077-7|
|Appare nelle tipologie:||01 - Articolo su periodico|