Monosomy 7 and deletion of the long arm of chromosome 7 are frequent non-random findings in myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML), moreover they are present in almost 50% of therapy-related myelodysplasia and therapy-related leukemia (t-MDS and t-AML). We used polymerase chain reaction (PCR) and Southern blotting and hybridization for restriction fragment length polymorphism (RFLP) analysis of the chromosomal region 7q22-7q31.3, where mostly losses of heterozigosity (LOH) have been described in MDS and t-AML. We studied 50 patients affected by MDS and t-AML for LOH in this region. We found LOH in 4 patients (8%), the deletions were found in 7q22.1 for one patient and in 7q31.1 for three more patients.
Deletion of long arm of chromosome 7 in myelodisplastic syndromes and therapy related acute myeloid leukemias / M. Bignotto, D. Cigognini, G. Corneo, P. Tripputi. - In: INTERNAL MEDICINE. CLINICAL AND LABORATORY. - ISSN 1590-9271. - 9:2(2001), pp. 45-48.
|Titolo:||Deletion of long arm of chromosome 7 in myelodisplastic syndromes and therapy related acute myeloid leukemias|
BIGNOTTO, MONICA (Primo)
TRIPPUTI, PASQUALE (Ultimo)
|Parole Chiave:||Chromosome 7; Deletion; DNA; Loss of heterozygosity; Myelodysplasia; Therapy related acute myeloid leukemia|
|Settore Scientifico Disciplinare:||Settore MED/09 - Medicina Interna|
Settore MED/03 - Genetica Medica
|Data di pubblicazione:||2001|
|Appare nelle tipologie:||01 - Articolo su periodico|