We evaluated the chemotactic activity of beta-endorphin and beta-endorphin-related peptides on human monocytes. We tested beta-endorphin(1-31) and fragments (1-16), (1-17), (1-27) in which the N-terminal of the opioid is preserved, N-acetyl-beta-endorphin(1-31) and fragments (6-31) and (28-31) in which the C-terminal is preserved, and fragment (2-17) that lacks both the N- and C-terminal. The fragments in which the N- and C-terminal were preserved [with the exception of fragment (28-31)] showed a chemotactic effect, while the lack of both terminals deprived the peptides of any activity. Moreover, only the N-terminal-mediated effects were naloxone reversible, while the C-terminal effects were not. These results indicate that while the intact N-terminal is necessary for opioid like effects, both N- and C-terminal can mediate effects on the immune system, thus offering evidence for a nonopioid receptor-mediated effect of opioid peptides on the immune system.
Analysis of the beta-endorphin structure-related activity on human monocyte chemotaxis: importance of the N- and C-terminal / P. SACERDOTE, A.E.PANERAI. - In: PEPTIDES. - ISSN 0196-9781. - 10:3(1989), pp. 565-569.
Titolo: | Analysis of the beta-endorphin structure-related activity on human monocyte chemotaxis: importance of the N- and C-terminal | |
Autori: | SACERDOTE, PAOLA GIUSEPPINA (Primo) PANERAI, ALBERTO EMILIO (Ultimo) | |
Parole Chiave: | Beta-endorphin; Beta-endorphin C-terminal fragments; Beta-endorphin N-terminal fragments; Chemotaxis; Immune system; Monocytes; Opioid receptors | |
Settore Scientifico Disciplinare: | Settore BIO/14 - Farmacologia | |
Data di pubblicazione: | 1989 | |
Rivista: | ||
Tipologia: | Article (author) | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/0196-9781(89)90143-5 | |
Appare nelle tipologie: | 01 - Articolo su periodico |