We recently found that long-term exposure to nitric oxide (NO) triggers mitochondrial biogenesis in mammalian cells and tissues by activation of guanylate cyclase and generation of cGMP. Here, we report that the NO/cGMP-dependent mitochondrial biogenesis is associated with enhanced coupled respiration and content of ATIP in U937, L6, and PC12 cells. The observed increase in ATIP content depended entirely on oxidative phosphorylation, because ATP formation by glycolysis was unchanged. Brain, kidney, liver, heart, and gastrocnemius muscle from endothelial NO synthase null mutant mice displayed markedly reduced mitochondrial content associated with significantly lower oxygen consumption and ATIP content. In these tissues, ultrastructural analyses revealed significantly smaller mitochondria. Furthermore, a significant reduction in the number of mitochondria was observed in the subsarcolemnal region of the gastrocnemius muscle. We conclude that NO/cGMP stimulates mitochondrial biogenesis, both in vitro and in vivo, and that this stimulation is associated with increased mitochondrial function, resulting in enhanced formation of ATIP
Reversible transdifferentiation of secretory epithelial cells into adipocytes in the mammary gland / M. Morroni, A. Giordano, M.C. Zingaretti, R. Boiani, R. De Matteis, B.B. Kahn, E. Nisoli, C. Tonello, C. Pisoschi, M.M. Luchetti, M. Marelli, S. Cinti. - In: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. - ISSN 0027-8424. - 101:48(2004), pp. 16801-16806.
|Titolo:||Reversible transdifferentiation of secretory epithelial cells into adipocytes in the mammary gland|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||2004|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1073/pnas.0407647101|
|Appare nelle tipologie:||01 - Articolo su periodico|