A new simplified radioassay for measuring polymorphonuclear leukocyte (PMN) chemotaxis is proposed using 51Cr-labeled cells and a single-filter system. The technique offers all the advantages described for the double-filter radioassay and permits a reproducible measurement of random locomotion, chemokinesis and chemotaxis. Moreover the single-filter radioassay utilizes commercially available and disposable chambers gathered in a multichamber apparatus; this makes the method very easy to learn and rapid to perform.
A new simplified single-filter assay for "in vitro" evaluation of chemotaxis of 51Cr-labeled polymorphonuclear leukocytes / F. Capsoni, F. Minonzio, A.M. Ongari, C. Zanussi. - In: JOURNAL OF IMMUNOLOGICAL METHODS. - ISSN 0022-1759. - 120:1(1989), pp. 125-131.
Titolo: | A new simplified single-filter assay for "in vitro" evaluation of chemotaxis of 51Cr-labeled polymorphonuclear leukocytes |
Autori: | CAPSONI, FRANCO (Primo) ONGARI, ANNA MARIA (Penultimo) |
Parole Chiave: | 51Cr assay; Chemotaxis; Polymorphonuclear leukocyte |
Settore Scientifico Disciplinare: | Settore MED/16 - Reumatologia |
Data di pubblicazione: | 1989 |
Rivista: | |
Tipologia: | Article (author) |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/0022-1759(89)90297-4 |
Appare nelle tipologie: | 01 - Articolo su periodico |