A new simplified radioassay for measuring polymorphonuclear leukocyte (PMN) chemotaxis is proposed using 51Cr-labeled cells and a single-filter system. The technique offers all the advantages described for the double-filter radioassay and permits a reproducible measurement of random locomotion, chemokinesis and chemotaxis. Moreover the single-filter radioassay utilizes commercially available and disposable chambers gathered in a multichamber apparatus; this makes the method very easy to learn and rapid to perform.
A new simplified single-filter assay for "in vitro" evaluation of chemotaxis of 51Cr-labeled polymorphonuclear leukocytes / F. Capsoni, F. Minonzio, A.M. Ongari, C. Zanussi. - In: JOURNAL OF IMMUNOLOGICAL METHODS. - ISSN 0022-1759. - 120:1(1989), pp. 125-131.
A new simplified single-filter assay for "in vitro" evaluation of chemotaxis of 51Cr-labeled polymorphonuclear leukocytes
F. CapsoniPrimo
;A.M. OngariPenultimo
;
1989
Abstract
A new simplified radioassay for measuring polymorphonuclear leukocyte (PMN) chemotaxis is proposed using 51Cr-labeled cells and a single-filter system. The technique offers all the advantages described for the double-filter radioassay and permits a reproducible measurement of random locomotion, chemokinesis and chemotaxis. Moreover the single-filter radioassay utilizes commercially available and disposable chambers gathered in a multichamber apparatus; this makes the method very easy to learn and rapid to perform.
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