Introduction: Hair testing has a leading role in toxicology practice and even more in those aspects tightly linked to the assessment of psychoactive drug use and abuse in social life. Aim: The objective of the present study was to develop and validate an automated SPE sample-preparation step, suited for GC/MS confirmation analysis of basic drugs in hair drug control. The method was studied and optimized for quantitative determination and in a second time it was extended to real hair samples. The purpose of method validation was to ensure good reliability, reproducibility and quickness. Methods: Janus Automated Workstation (PerkinElmer) was employed to perform SPE hair extraction, using 96-well plate SPEC MP1 acquired from Varian (Agilent Technologies). After derivatization of dried extracts, screening confirmations were performed using gas chromatography (GC) followed by mass spectrometry (MS). GC/MS data were validated following standard guidelines, but our attention was focused on three headings: samples cross-contamination, "memory effect" and extraction recovery. Results: Validation requests were fully accomplished and we always obtained best results with the automated procedure. For instance, analytes mean recovery was between 70 and 90% and data analysis proved that no contamination between samples occurred. Conclusions: The automated workstation has shown good reliability (cross contamination and "memory effect" were tested and excluded), effectiveness (no false negative was detected), solvent saving (500 μL/sample vs traditionally LLE 4. mL/sample) and quickness (50. min for 96 tests cycle).
Automated fast procedure for the simultaneous extraction of hair sample performed with an automated workstation / I. Angeli, M. Minoli, A. Ravelli, F. Gigli, F. Lodi. - In: FORENSIC SCIENCE INTERNATIONAL. - ISSN 0379-0738. - 218:1-3(2012), pp. 15-19.
|Titolo:||Automated fast procedure for the simultaneous extraction of hair sample performed with an automated workstation|
ANGELI, ILARIA (Primo)
MINOLI, MAURO (Secondo)
GIGLI, FAUSTO (Penultimo)
LODI, FRANCO (Ultimo)
|Parole Chiave:||Automated SPE; Drugs; Hair|
|Settore Scientifico Disciplinare:||Settore MED/43 - Medicina Legale|
|Data di pubblicazione:||2012|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.forsciint.2011.10.008|
|Appare nelle tipologie:||01 - Articolo su periodico|