A fully automated fluorometer which measures fluorescence depolarization is described. The instrument has been designed to work with standard microtiter sample holders normally used in clinical tests. The combination of a hybrid optical system, built with discrete optical elements and fiber bundles, and a digital Fourier analysis of the detected signals make the instrument quite sensitive and precise. The 96 samples are scanned in about 100 s, with a reproducibility of the measurement of the polarization ratio of about 10(-3) and a minimum content of fluorescein in the sample well of 20 pmol

A sensitive polaryzed fluorometer for clinical tests / P. Lago, L. Rovati, M. Corti. - In: IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT. - ISSN 0018-9456. - 43:4(1994), pp. 664-667.

A sensitive polaryzed fluorometer for clinical tests

M. Corti
Ultimo
1994

Abstract

A fully automated fluorometer which measures fluorescence depolarization is described. The instrument has been designed to work with standard microtiter sample holders normally used in clinical tests. The combination of a hybrid optical system, built with discrete optical elements and fiber bundles, and a digital Fourier analysis of the detected signals make the instrument quite sensitive and precise. The 96 samples are scanned in about 100 s, with a reproducibility of the measurement of the polarization ratio of about 10(-3) and a minimum content of fluorescein in the sample well of 20 pmol
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/20187
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