We have performed a physiological analysis of the effects of high- osmolarity media on gas1Δ cells. The reductions in the duplication time, number of pluribudded cells, hypersensitivity to Calcofluor and sodium dodecyl sulfate, and chitin level indicate a partial suppression of the mutant phenotype. GAS1 deletion was found to be lethal in the absence of the Bck1 and Slt2 (Mpk1) proteins of the cell integrity pathway.

Increase of external osmolarity reduces morphogenetic defects and accumulation of chitin in a gas1 mutant of Saccharomyces cerevisiae / A. Turchini, L. Ferrario, L. Popolo. - In: JOURNAL OF BACTERIOLOGY. - ISSN 0021-9193. - 182:4(2000), pp. 1167-1171. [10.1128/JB.182.4.1167-1171.2000]

Increase of external osmolarity reduces morphogenetic defects and accumulation of chitin in a gas1 mutant of Saccharomyces cerevisiae

L. Popolo
Ultimo
2000

Abstract

We have performed a physiological analysis of the effects of high- osmolarity media on gas1Δ cells. The reductions in the duplication time, number of pluribudded cells, hypersensitivity to Calcofluor and sodium dodecyl sulfate, and chitin level indicate a partial suppression of the mutant phenotype. GAS1 deletion was found to be lethal in the absence of the Bck1 and Slt2 (Mpk1) proteins of the cell integrity pathway.
Settore BIO/11 - Biologia Molecolare
Settore BIO/19 - Microbiologia Generale
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/200604
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