The biochemical nature of the start process that commits budding yeast to DNA synthesis is not known. Kinetic evidence has suggested recently that short-lived protein(s) may have to accumulate to a critical level before the cell cycle may progress towards DNA synthesis and cell division. We investigated by high-reslution two-dimensional electrophoresis whether, in a cdc25-1 mutant strain of Saccharomyces cerevisiae that had been blocked at the regulatory step called 'start' by growth at a restrictive temperature, short-lived proteins are synthesized during the recovery of growth at a permissive temperature. Of the ~500 proteins resolved by the two-dimensional electrophoresis, 6 were short-lived. Only one of them (M(r) = 100,000 pI ~ 4.8-5) appears to be specifically made during the G1-to-S transition at start. A regulatory role for cell cycle progression in yeast is suggested for this protein, p100.
|Titolo:||Identification of a labile protein involved in the G1 to S transition in Saccharomyces cerevisiae|
POPOLO, LAURA MARIA (Primo)
|Settore Scientifico Disciplinare:||Settore BIO/11 - Biologia Molecolare|
Settore BIO/10 - Biochimica
|Data di pubblicazione:||1984|
|Appare nelle tipologie:||01 - Articolo su periodico|