We have examined the allogeneic mixed lymphocyte reaction (MLR) and autologous mixed lymphocyte reaction (AMLR) stimulating activities of T cells precultured in vitro with soluble allogeneic or autologous HLA-DR antigens. These cells (Ts) are known to suppress the human MLR: this suppression is specific in that it occurs only when stimulator cells have the same HLA-DR antigen as that used to induce differentiation of suppressor cells. Ts cells express new membrane specificities; they can be separated by immunoabsorption into two populations: Ts enriched (Tx+; with suppressive activity) and Ts depleted (Ts-; with helper function). In the present study, we have demonstrated that both Ts cell subsets activated by soluble HLA-DR alloantigens are able to stimulate both MLR and AMLR. Ts cells activated by soluble autologous HLA-DR antigens are able to stimulate MLR, but not AMLR.

AMLR and MLR stimulating activity of human T lymphocytes activated in vitro by soluble HLA-DR antigens / M. Vanoli, R. Scorza Smeraldi, G. Fabio, P. Bonara, M. G. Sabbadini, C. Zanussi. - In: CLINICAL AND EXPERIMENTAL IMMUNOLOGY. - ISSN 0009-9104. - 63:1(1986 Jan), pp. 163-70-170.

AMLR and MLR stimulating activity of human T lymphocytes activated in vitro by soluble HLA-DR antigens

R. Scorza Smeraldi;G. Fabio;
1986-01

Abstract

We have examined the allogeneic mixed lymphocyte reaction (MLR) and autologous mixed lymphocyte reaction (AMLR) stimulating activities of T cells precultured in vitro with soluble allogeneic or autologous HLA-DR antigens. These cells (Ts) are known to suppress the human MLR: this suppression is specific in that it occurs only when stimulator cells have the same HLA-DR antigen as that used to induce differentiation of suppressor cells. Ts cells express new membrane specificities; they can be separated by immunoabsorption into two populations: Ts enriched (Tx+; with suppressive activity) and Ts depleted (Ts-; with helper function). In the present study, we have demonstrated that both Ts cell subsets activated by soluble HLA-DR alloantigens are able to stimulate both MLR and AMLR. Ts cells activated by soluble autologous HLA-DR antigens are able to stimulate MLR, but not AMLR.
Histocompatibility Antigens Class II; T-Lymphocytes, Regulatory; Solubility; Humans; Lymphocyte Culture Test, Mixed; Lymphocyte Activation; HLA-DR5 Antigen; Cells, Cultured; HLA-DR Antigens; Adult; HLA-DR7 Antigen; Epitopes; T-Lymphocytes
Settore MED/09 - Medicina Interna
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/200515
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