The detection of human chromosomes in somatic cell hybrids is usually made by chromosomal analysis, Southern blot analysis with human probes, and starch-gel electrophoresis of isoenzymes. We describe here a new, quick, and very efficient method to detect human chromosomes in somatic cell hybrids between human and rodent (rat and mouse) cells. The method is based on the polymerase chain reaction to promote amplification of human DNA, using primers derived from localized genes or DNA fragments from each human chromosome.
Detection of human chromosomes in somatic cell hybrids by PCR analysis / P. Tripputi, G. Bacciocchi, R.S. Accolla, G. Mantero, G. De Bellis, M. Manoni, G. Cassani. - In: CYTOGENETICS AND CELL GENETICS. - ISSN 0301-0171. - 62:1(1993), pp. 1-4. [10.1159/000133432]
Detection of human chromosomes in somatic cell hybrids by PCR analysis
P. TripputiPrimo
;
1993
Abstract
The detection of human chromosomes in somatic cell hybrids is usually made by chromosomal analysis, Southern blot analysis with human probes, and starch-gel electrophoresis of isoenzymes. We describe here a new, quick, and very efficient method to detect human chromosomes in somatic cell hybrids between human and rodent (rat and mouse) cells. The method is based on the polymerase chain reaction to promote amplification of human DNA, using primers derived from localized genes or DNA fragments from each human chromosome.
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