Human T lymphocytes precultured for 36 hr in the presence of soluble HLA-DR antigens suppress the MLR response of autologous peripheral blood lymphocytes to allogeneic stimulating cells. The suppression is DR antigen-specific in that it appears that the MLR stimulating cell donor and the soluble suppressor-inducing antigen must share DR specificities. The soluble DR antigens were fractionated from the sera of normal donors using QAE-Sephadex chromatography and CNBr-activated Sepharose immunoadsorption. Similarly prepared HLA-A and -B antigens failed to induce suppressive activity. The suppressive activity of DR-antigen cultured T cells is resistant to mitomycin C treatment and, further, the antigen specificity is maintained with or without mitomycin C treatment. The kinetics of suppressor cell induction as well as the kinetics of suppression in the test MLR cultures are presented. The implications of these results are discussed.
|Titolo:||MLC-specific suppressor T lymphocytes in man. I. Their induction in vitro by soluble HLA-DR antigens|
FABIO, GIOVANNA (Secondo)
|Parole Chiave:||Histocompatibility Antigens Class II; T-Lymphocytes, Regulatory; Solubility; Kinetics; Humans; Lymphocyte Culture Test, Mixed; Adult; Mitomycins; Epitopes; T-Lymphocytes|
|Settore Scientifico Disciplinare:||Settore MED/09 - Medicina Interna|
|Data di pubblicazione:||mar-1982|
|Appare nelle tipologie:||01 - Articolo su periodico|