The aggregative properties of highly pure GM3 ganglioside in aqueous solution have been studied by static and dynamic laser light scattering measurements and fluorescence spectroscopy experiments performed by the use of a GM3 derivative containing the pyrene group at the end of the fatty acid moiety. GM3 ganglioside spontaneously aggregates as unilamellar vesicles, down to a concentration of 1.25 × 10-8 M, showing molecular weight and hydrodynamic radius ranging from 15,000 to 30,000 kDa and from 350 to 470 Å, respectively. GM3 vesicles are stable with dilution and can be stored at room temperature for some weeks without appreciable change.

Aggregation Properties of GM3 Ganglioside (II3Neu5AcLacCer) in Aqueous Solutions / S. SONNINO, L. CANTU, D. ACQUOTTI, M. CORTI, G. TETTAMANTI. - In: CHEMISTRY AND PHYSICS OF LIPIDS. - ISSN 0009-3084. - 52:3-4(1990), pp. 231-241.

Aggregation Properties of GM3 Ganglioside (II3Neu5AcLacCer) in Aqueous Solutions

S. SONNINO
Primo
;
L. CANTU
Secondo
;
M. CORTI
Penultimo
;
G. TETTAMANTI
Ultimo
1990

Abstract

The aggregative properties of highly pure GM3 ganglioside in aqueous solution have been studied by static and dynamic laser light scattering measurements and fluorescence spectroscopy experiments performed by the use of a GM3 derivative containing the pyrene group at the end of the fatty acid moiety. GM3 ganglioside spontaneously aggregates as unilamellar vesicles, down to a concentration of 1.25 × 10-8 M, showing molecular weight and hydrodynamic radius ranging from 15,000 to 30,000 kDa and from 350 to 470 Å, respectively. GM3 vesicles are stable with dilution and can be stored at room temperature for some weeks without appreciable change.
ganglioside aggregates; ganglioside derivatives; gangliosides; light scattering; vesicles
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
Settore BIO/10 - Biochimica
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/200296
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