Two caprine herpesvirus (CpHV.1) strains isolated in two goat flocks in southern Italy were compared with E/CH and McK/US reference CpHV.1 strains by restriction endonuclease analysis. BamHI, KpnI, NotI and PstI restriction enzymes were used. With these enzymes McK/US strain was clearly differentiated from the others. KpnI and NotI digestion resulted in unique patterns for all four strains. Restriction enzyme analysis might be a useful method to distinguish CpHV.1 isolated from animals and provide a basis for further epidemiological studies of the CpHV.1 infection.
Restriction endonuclease analysis of the genome of two Italian caprine herpesvirus 1 strains / A. Pratelli, G. Greco, P. Dall'Ara, M. Engels, M. Tempesta, C. Buonavoglia. - In: ARCHIVES OF VIROLOGY. - ISSN 0304-8608. - 145:4(2000), pp. 845-851.
Restriction endonuclease analysis of the genome of two Italian caprine herpesvirus 1 strains
P. Dall'Ara;
2000
Abstract
Two caprine herpesvirus (CpHV.1) strains isolated in two goat flocks in southern Italy were compared with E/CH and McK/US reference CpHV.1 strains by restriction endonuclease analysis. BamHI, KpnI, NotI and PstI restriction enzymes were used. With these enzymes McK/US strain was clearly differentiated from the others. KpnI and NotI digestion resulted in unique patterns for all four strains. Restriction enzyme analysis might be a useful method to distinguish CpHV.1 isolated from animals and provide a basis for further epidemiological studies of the CpHV.1 infection.
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