Retinoic acid (RA) reduced growth, fibronectin, and retinoic acid receptor (RAR alpha) in NIH 3T3 cells but not in cells transformed by the Ha-ras oncogene. RA lowered RAR alpha transcript and protein, increased RAR beta transcripts, and had no effect on RAR gamma. H-ras transformation downregulated RAR expression and abolished responsiveness to RA. Ha-ras-transformed cells were as active as normal NIH-3T3 cells in RA uptake but were unable to degrade it to medium oxidation product, so that, paradoxically, the resistant cells accumulated 20-30-fold as much RA as the sensitive cells. RA sensitivity/insensitivity correlated with RA metabolism/lack thereof in 15 cell lines in serum-free medium. These data suggest a relationship between RA inhibition of cell growth and intracellular RA metabolism.
|Titolo:||Retinoic acid downregulates growth, fibronectin and RAR alpha in 3T3 cells: Ha-ras blocks this response and RA metabolism|
SCITA, GIORGIO (Secondo)
|Parole Chiave:||Genes, ras; Animals; 3T3 Cells; Receptors, Retinoic Acid; Fibronectins; Tretinoin; Mice; Cell Division|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||nov-1997|
|Digital Object Identifier (DOI):||10.1002/(SICI)1097-4652(199711)173:2<297::AID-JCP39>3.0.CO;2-A|
|Appare nelle tipologie:||01 - Articolo su periodico|