The extracellular form of the K5 polysaccharide was produced, purified and characterized from a strain of Escherichia coli, the maximum fermentation yield (320 mg/L) was obtained after 15 h. The polysaccharide, recovered by precipitation with cold ethanol (80%), was purified through a process employing an enzymic deproteination step using fungal protease. Characterization by TLC and GC chromatography revealed that the K5 was mainly composed of glucuronic acid and N-acetyl-glucosamine. The C-13-NMR spectrum showed the product's structure to be the same as that of the K5 capsular polysaccharide described in the literature.
EXTRACELLULAR K5 POLYSACCHARIDE OF ESCHERICHIA-COLI - PRODUCTION AND CHARACTERIZATION / M. MANZONI, S. BERGOMI, V. CAVAZZONI. - In: JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS. - ISSN 0883-9115. - 8:3(1993), pp. 251-257. [10.1177/088391159300800304]
EXTRACELLULAR K5 POLYSACCHARIDE OF ESCHERICHIA-COLI - PRODUCTION AND CHARACTERIZATION
M. ManzoniPrimo
;
1993
Abstract
The extracellular form of the K5 polysaccharide was produced, purified and characterized from a strain of Escherichia coli, the maximum fermentation yield (320 mg/L) was obtained after 15 h. The polysaccharide, recovered by precipitation with cold ethanol (80%), was purified through a process employing an enzymic deproteination step using fungal protease. Characterization by TLC and GC chromatography revealed that the K5 was mainly composed of glucuronic acid and N-acetyl-glucosamine. The C-13-NMR spectrum showed the product's structure to be the same as that of the K5 capsular polysaccharide described in the literature.
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