The extracellular form of the K5 polysaccharide was produced, purified and characterized from a strain of Escherichia coli, the maximum fermentation yield (320 mg/L) was obtained after 15 h. The polysaccharide, recovered by precipitation with cold ethanol (80%), was purified through a process employing an enzymic deproteination step using fungal protease. Characterization by TLC and GC chromatography revealed that the K5 was mainly composed of glucuronic acid and N-acetyl-glucosamine. The C-13-NMR spectrum showed the product's structure to be the same as that of the K5 capsular polysaccharide described in the literature.
|Titolo:||EXTRACELLULAR K5 POLYSACCHARIDE OF ESCHERICHIA-COLI - PRODUCTION AND CHARACTERIZATION|
MANZONI, MATILDE (Primo)
|Settore Scientifico Disciplinare:||Settore AGR/16 - Microbiologia Agraria|
|Data di pubblicazione:||1993|
|Digital Object Identifier (DOI):||10.1177/088391159300800304|
|Appare nelle tipologie:||01 - Articolo su periodico|