Extracellular inulinase from Kluyveromyces marxianus var. bulgaricus catalysed the hydrolysis of pure inulin and the extracts of fresh and dried topinambur (Jerusalem artichoke). At an enzyme concentration of 10 IU g-1 of substrate the three substrates were hydrolysed respectively to 65.3, 77.3 and 83.9%. The relationship between the extent of hydrolysis, reaction time and enzyme concentration was studied and a kinetic model of hydrolysis was derived.
HYDROLYSIS OF TOPINAMBUR (JERUSALEM-ARTICHOKE) FRUCTANS BY EXTRACELLULAR INULINASE OF KLUYVEROMYCES-MARXIANUS VAR BULGARICUS / M. MANZONI, V. CAVAZZONI. - In: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY. - ISSN 0268-2575. - 54:4(1992), pp. 311-315.
HYDROLYSIS OF TOPINAMBUR (JERUSALEM-ARTICHOKE) FRUCTANS BY EXTRACELLULAR INULINASE OF KLUYVEROMYCES-MARXIANUS VAR BULGARICUS
M. MANZONIPrimo
;
1992
Abstract
Extracellular inulinase from Kluyveromyces marxianus var. bulgaricus catalysed the hydrolysis of pure inulin and the extracts of fresh and dried topinambur (Jerusalem artichoke). At an enzyme concentration of 10 IU g-1 of substrate the three substrates were hydrolysed respectively to 65.3, 77.3 and 83.9%. The relationship between the extent of hydrolysis, reaction time and enzyme concentration was studied and a kinetic model of hydrolysis was derived.
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