A rapid and simple method for evaluating the efficiency of DNA labelling reactions with haptens is described. The method, called the Flow-Through Hapten-DNA Assay (FT-HDA), relies on binding of anti-hapten antibodies/alkaline phosphatase conjugates to hapten-DNA, immobilized on disposable capillary absorbent filters, and visual detection of blue-grey coloured spots appearing on the filter after chromogenic reaction with enzyme substrates. FT-HDA of hapten-DNA is markedly faster and simpler than conventional diffusion assays on membranes.

Rapid detection of hapten-labeled DNA by flow-through dot immunobinding assay / A. Corti, P. Tripputi, G. Cassani. - In: JOURNAL OF IMMUNOLOGICAL METHODS. - ISSN 0022-1759. - 134:1(1990), pp. 81-86.

Rapid detection of hapten-labeled DNA by flow-through dot immunobinding assay

P. Tripputi
Secondo
;
1990

Abstract

A rapid and simple method for evaluating the efficiency of DNA labelling reactions with haptens is described. The method, called the Flow-Through Hapten-DNA Assay (FT-HDA), relies on binding of anti-hapten antibodies/alkaline phosphatase conjugates to hapten-DNA, immobilized on disposable capillary absorbent filters, and visual detection of blue-grey coloured spots appearing on the filter after chromogenic reaction with enzyme substrates. FT-HDA of hapten-DNA is markedly faster and simpler than conventional diffusion assays on membranes.
Settore MED/04 - Patologia Generale
1990
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/198453
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