Either aphidicolin- or thymidine-synchronized human HL-60 cells were used to study the replication pattern of a family of human repetitive DNA sequences, the Eco RI 340 bp family (alpha RI-DNA), and of the ladders of fragments generated in total human DNA after digestion with XbaI and HaeIII (alpha satellite sequences). DNAs replicated in early, middle-early, middle-late and late S periods were labelled with BUdR or with [3H]thymidine. The efficiency of the cell synchronization procedure was confirmed by the transition from a high-GC to a high-AT average base composition of the DNA synthesized going from early to late S periods. By hybridizing EcoRI 340 bp repetitive fragments to BUdR-DNAs it was found that this family of sequences is replicated throughout the entire S period. Comparing fluorograph densitometric scans of [3H]DNAs to the scans of ethidium bromide patterns of total HL-60 DNA digested with XbaI and HaeIII, it was observed that DNA synthesized in different S periods is characterized by approximately the same ladder of fragments, while the intensity of each band may vary through the S phase; in particular, the XbaI 2.4 kb fragment becomes undetectable in late S.
Replication pattern of human repeated DNA sequences / R. Meneveri, A. Agresti, D. Breviario, E. Ginelli. - In: BIOCHIMICA ET BIOPHYSICA ACTA. - ISSN 0006-3002. - 783:1(1984 Oct 05), pp. 53-59.
|Titolo:||Replication pattern of human repeated DNA sequences|
GINELLI, ENRICO (Ultimo)
|Parole Chiave:||Cell cycle; DNA replication; repetitive DNA|
|Settore Scientifico Disciplinare:||Settore BIO/13 - Biologia Applicata|
|Data di pubblicazione:||5-ott-1984|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/0167-4781(84)90077-0|
|Appare nelle tipologie:||01 - Articolo su periodico|