Fluorescence in situ hybridization (FISH) using chromosome specific alpha-satellite DNA probes for chromosome 7, was performed on paraffin-embedded sections of 21 cases of human "unstable" atherosclerotic plaques, in order to determine whether trisomy 7, found by some Authors with conventional cytogenetics, is a biological characteristic of the atherosclerotic plaque. In 15 cases (71%) the FISH method showed smooth muscle cells with three or more spots. In particular the trisomy 7 was the most significant and constant result. The presence of an extra chromosome 7 is strictly correlated with an overexpression of the gene for chain A of the platelet-derived growth factor (PDGF), which was mapped at pter-7q22 region. Therefore trisomy 7 seems to correspond to an increase in PDGF synthesis and consequently to an increase in proliferative activity of smooth muscle cells.
Cytogenetic aspects of cell proliferation in atherosclerotic plaques / L. Matturri, A. Cazzullo, P. Turconi, A.M. Lavezzi. - In: CARDIOLOGIA. - ISSN 0393-1978. - 42:8(1997 Aug), pp. 833-836.
Cytogenetic aspects of cell proliferation in atherosclerotic plaques
L. MatturriPrimo
;A.M. LavezziUltimo
1997
Abstract
Fluorescence in situ hybridization (FISH) using chromosome specific alpha-satellite DNA probes for chromosome 7, was performed on paraffin-embedded sections of 21 cases of human "unstable" atherosclerotic plaques, in order to determine whether trisomy 7, found by some Authors with conventional cytogenetics, is a biological characteristic of the atherosclerotic plaque. In 15 cases (71%) the FISH method showed smooth muscle cells with three or more spots. In particular the trisomy 7 was the most significant and constant result. The presence of an extra chromosome 7 is strictly correlated with an overexpression of the gene for chain A of the platelet-derived growth factor (PDGF), which was mapped at pter-7q22 region. Therefore trisomy 7 seems to correspond to an increase in PDGF synthesis and consequently to an increase in proliferative activity of smooth muscle cells.Pubblicazioni consigliate
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