The gp51-p30 glycoprotein constituting BLV envelope was expressed in Sf-21 insect cells by means of recombinant baculoviruses. Post-infection cell lysates mere analyzed, in order to define the immunologic reactivity of recombinant products. Oligosaccharide chains, containing N-acetylglucosamine, mannose, galactose and sialic acid mere found on recombinant gp51-p30. In order to investigate the timing of transcription and translation of the glycoprotein, kinetic assays were carried out on cell lysates and directly in situ on Sf-21 cells during the course of baculovirus infection, The use of different solubilizing reagents mas also evaluated in order to rescue recombinant glycoprotein from its subcellular location.
Expression of bovine leukemia virus ENV glycoprotein in insect cells by recombinant baculovirus / S. Russo, L. Montermini, T. Berkovitz-Siman, W. Ponti, G. Poli. - In: FEBS LETTERS. - ISSN 0014-5793. - 436:1(1998 Sep), pp. 11-16.
Expression of bovine leukemia virus ENV glycoprotein in insect cells by recombinant baculovirus
G. PoliUltimo
1998
Abstract
The gp51-p30 glycoprotein constituting BLV envelope was expressed in Sf-21 insect cells by means of recombinant baculoviruses. Post-infection cell lysates mere analyzed, in order to define the immunologic reactivity of recombinant products. Oligosaccharide chains, containing N-acetylglucosamine, mannose, galactose and sialic acid mere found on recombinant gp51-p30. In order to investigate the timing of transcription and translation of the glycoprotein, kinetic assays were carried out on cell lysates and directly in situ on Sf-21 cells during the course of baculovirus infection, The use of different solubilizing reagents mas also evaluated in order to rescue recombinant glycoprotein from its subcellular location.
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