The gp51-p30 glycoprotein constituting BLV envelope was expressed in Sf-21 insect cells by means of recombinant baculoviruses. Post-infection cell lysates mere analyzed, in order to define the immunologic reactivity of recombinant products. Oligosaccharide chains, containing N-acetylglucosamine, mannose, galactose and sialic acid mere found on recombinant gp51-p30. In order to investigate the timing of transcription and translation of the glycoprotein, kinetic assays were carried out on cell lysates and directly in situ on Sf-21 cells during the course of baculovirus infection, The use of different solubilizing reagents mas also evaluated in order to rescue recombinant glycoprotein from its subcellular location.
|Titolo:||Expression of bovine leukemia virus ENV glycoprotein in insect cells by recombinant baculovirus|
POLI, GIORGIO (Ultimo)
|Parole Chiave:||baculovirus ; biotechnology ; bovine leukemia virus ; glycosylation ; recombinant glycoprotein|
|Settore Scientifico Disciplinare:||Settore VET/05 - Malattie Infettive degli Animali Domestici|
|Data di pubblicazione:||set-1998|
|Digital Object Identifier (DOI):||10.1016/S0014-5793(98)00951-X|
|Appare nelle tipologie:||01 - Articolo su periodico|