Light and heavy lysosomes of mouse forebrain were separated from each other by centrifugation on a Percoll gradient. Light lysosomes were then freed from mitochondria and membranes by sucrose density gradient centrifugation and further purified by floatation-centrifugation on a sucrose gradient. The final preparations of light and heavy lysosomes, fairly homogenous, carried sinlidase activity, assayed on MU-NeuAc. The optimal pH was 4.0 and 4.2, the apparent Km value 2.8 × 10-3 M and 4.2 × 10-3 M and the apparent Vmax value 0.11 and 0.47 mU mg-1 protein, for the light and heavy lysosome sialidase, respectively. From 4 days to adulthood the specific activity of the light and heavy lysosome sialidase increased 3-fold and 1.7-fold, respectively.
occurrence of sialidase activity in two distinct and highly homogeneous populations of lysosomes prepared from the brain of developing mouse / A. Fiorilli, C. Siniscalco, A. Chiarini, L. Di Francesco, B. Venerando, G. Tettamanti. - In: FEBS LETTERS. - ISSN 0014-5793. - 282:2(1991 May), pp. 235-238.
|Titolo:||occurrence of sialidase activity in two distinct and highly homogeneous populations of lysosomes prepared from the brain of developing mouse|
FIORILLI, AMELIA (Primo)
VENERANDO, BRUNO (Penultimo)
|Parole Chiave:||Brain development; Lysosome; Sialidase; Subcellular fractionation.|
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||mag-1991|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/0014-5793(91)80485-L|
|Appare nelle tipologie:||01 - Articolo su periodico|