In the leech, serotonin (5HT) is involved in several non associative leamingprocesses. It is thought that the role of5HT on short term learning is due to the activation of different protein kinases, such as protein kinase A and protein kinase C, and that new protein synthesis is involved in longterm learning. Behavioral experiments have shown that short-term learning processes are not affected by the use of protein synthesis inhibitors, whereas some forms of long.term learning are. In order to study the molecular mechanisms underlying such forms of learning we have analyzed the effects of SHT on 32P-onophosphoric acid and S35-L-methionine incorporation. The proteins have been separated by means of two dimensional-electrophoresis and detected by autoradiography. We have revealed the changes on protein phosphorylation produced by 5HT in the leech ganglia. In addition we mimiked the long term learning either by prolonged or by repetitive treatment of the ganglia with SHT. In both cases this neurotransmitter modifies the incorporation of 35S-L-methionine into some proteins. But the results obtained with the two different treatments are not identical. To know whether protein phosphorylation is necessary for the lction of srrr on protein synthesis. we are studyint; the effects of protein Kinase inhibitors on methionine incorporation during repetitive stimulation with 5HT. These experiments can enlight the cascade of molecular events which transduces the learning capabilities from short· term processes to the long·term ones.
Modulation of protein synthesis and protein phosphorylation induced by 5-hydroxytrptamine in the central nervous system of the leech Hirudo medicinalis / D. Bottai, M.L. Zaccardi, M. Garcia Gil, M. Brunelli. - In: JOURNAL OF NEUROCHEMISTRY. - ISSN 0022-3042. - 61:Suppl. 1(1993), pp. 94-94. ((Intervento presentato al 14. convegno Meeting of International Society of Neurochemistry tenutosi a Montpellier nel 1993.
Modulation of protein synthesis and protein phosphorylation induced by 5-hydroxytrptamine in the central nervous system of the leech Hirudo medicinalis
D. BottaiPrimo
;
1993
Abstract
In the leech, serotonin (5HT) is involved in several non associative leamingprocesses. It is thought that the role of5HT on short term learning is due to the activation of different protein kinases, such as protein kinase A and protein kinase C, and that new protein synthesis is involved in longterm learning. Behavioral experiments have shown that short-term learning processes are not affected by the use of protein synthesis inhibitors, whereas some forms of long.term learning are. In order to study the molecular mechanisms underlying such forms of learning we have analyzed the effects of SHT on 32P-onophosphoric acid and S35-L-methionine incorporation. The proteins have been separated by means of two dimensional-electrophoresis and detected by autoradiography. We have revealed the changes on protein phosphorylation produced by 5HT in the leech ganglia. In addition we mimiked the long term learning either by prolonged or by repetitive treatment of the ganglia with SHT. In both cases this neurotransmitter modifies the incorporation of 35S-L-methionine into some proteins. But the results obtained with the two different treatments are not identical. To know whether protein phosphorylation is necessary for the lction of srrr on protein synthesis. we are studyint; the effects of protein Kinase inhibitors on methionine incorporation during repetitive stimulation with 5HT. These experiments can enlight the cascade of molecular events which transduces the learning capabilities from short· term processes to the long·term ones.Pubblicazioni consigliate
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