Many neural functions are regulated by phosphorylation of proteins. In the leech, behavioral experiments have shown that SHT is involved in short-term non-associative learning through the activation of cAMP- dependent protein kinases. On the other hand, long term dishabituation requires new protein synthesis but the mechanisms underlying the passage from the short-term to the long-term learning are unknown. In order to study this issue, we have labeled ganglia with either (32P)- orthophosphoric add or (35S)-methionine. Labeled proteins were separated by bidimensional electrophoresis and detected by autoradiography. A single treatment with 5HT 5OµM was found to produce a general increase in protein phosphorylation. Repetitive treatments with 5HT induced a higher level of protein phosphorylation and a modification of (35)-methionine incorporation. Repetitive treatment with ooth 5HT and the protein kinase inhibitor staurosporine resulted in a 20-5O% inhibition of (35S)-methionine incorporation into proteins. These results suggest that protein phosphorylation is important to trigger protein synthesis, possibily mediating the passage from short-term to long-tenn modulation.
Repetitive treatment with serotonin induces changes in protein synthesis and protein phosphorylation in the CNS of the leech Hirudo medicinalis / D. Bottai, M. Garcia Gil, M. Brunelli. ((Intervento presentato al 24. convegno Society of Neuroscience Annual Meeting tenutosi a Miami nel 1994.
Repetitive treatment with serotonin induces changes in protein synthesis and protein phosphorylation in the CNS of the leech Hirudo medicinalis
D. BottaiPrimo
;
1994
Abstract
Many neural functions are regulated by phosphorylation of proteins. In the leech, behavioral experiments have shown that SHT is involved in short-term non-associative learning through the activation of cAMP- dependent protein kinases. On the other hand, long term dishabituation requires new protein synthesis but the mechanisms underlying the passage from the short-term to the long-term learning are unknown. In order to study this issue, we have labeled ganglia with either (32P)- orthophosphoric add or (35S)-methionine. Labeled proteins were separated by bidimensional electrophoresis and detected by autoradiography. A single treatment with 5HT 5OµM was found to produce a general increase in protein phosphorylation. Repetitive treatments with 5HT induced a higher level of protein phosphorylation and a modification of (35)-methionine incorporation. Repetitive treatment with ooth 5HT and the protein kinase inhibitor staurosporine resulted in a 20-5O% inhibition of (35S)-methionine incorporation into proteins. These results suggest that protein phosphorylation is important to trigger protein synthesis, possibily mediating the passage from short-term to long-tenn modulation.Pubblicazioni consigliate
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