Different Helicobacter pylori lipopolysaccharides (LPSs) and LPS-derivatives were studied for their ability to induce the production of procoagulant activity (PCA) and plasminogen activator inhibitor type 2 (PAI-2) by human blood mononuclear leukocytes. Smooth (S)- and rough (R)-form LPSs caused a similar increase in cell-associated PCA (tissue factor) and PAI-2 antigen release. Both effects were potentiated by fetal bovine serum via a CD14-mediated mechanism. The potency of H. pylori LPSs was approximately 1000-fold lower than that of Salmonella typhimurium LPSs. When H. pylori LPS derivatives (dephosphorylated R-LPS, S-lipid A, and R-lipid A) were used, PCA and PAI-2 production were markedly reduced. R-lipid A was approximately 4-fold less efficient than S-lipid A. These findings suggest that the induction of monocyte tissue factor and PAI-2 by H. pylori LPS is influenced by the lipid A structure and modulated by the core oligosaccharide and that phosphate groups present in both regions may play an important role.
Effect of Helicobacter pylori lipopolysaccharide (LPS) and LPS derivatives on the production of tissue factor and plasminogen activator inhibitor type 2 by human blood mononuclear cells / N. Semeraro, P. Montemurro, C. Piccoli, V. Muolo, M. Colucci, G. Giuliani, D. Fumarola, S. Pece, A. P. Moran. - In: THE JOURNAL OF INFECTIOUS DISEASES. - ISSN 0022-1899. - 174:6(1996 Dec), pp. 1255-60-1260.
|Titolo:||Effect of Helicobacter pylori lipopolysaccharide (LPS) and LPS derivatives on the production of tissue factor and plasminogen activator inhibitor type 2 by human blood mononuclear cells|
PECE, SALVATORE (Penultimo)
|Parole Chiave:||Helicobacter pylori; Leukocytes, Mononuclear; Thromboplastin; Humans; Lipopolysaccharides; Serum Albumin, Bovine; Plasminogen Activator Inhibitor 2; Helicobacter Infections; Salmonella typhimurium; Antigens, CD14|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||dic-1996|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1093/infdis/174.6.1255|
|Appare nelle tipologie:||01 - Articolo su periodico|