Sera from systemic lupus erythematosus (SLE) patients giving a fluorescent ribosomal pattern on tissue and cell preparations also showed precipitating autoantibodies against purified rat liver ribosomes. Ribosomal antigen is also present in rabbit thymus cellular extract (RTE), since the same sera gave precipitin lines against RTE in identity with ribosomes. Immunofluorescent staining was completely inhibited by serum absorption with ribosomes or with RTE. However ribosomal RNA and RNase or trypsin-treated ribosomes failed to react with these autoantibodies as demonstrated in immunoabsorption and immunodiffusion studies. These data suggest that these sera contain autoantibodies directed against some antigenic site composed of a portion of both RNA and ribosomal protein. Ribosomal autoantibodies were detectable at a low frequency in SLE patients characterized by an active disease and renal involvement.

Anti-ribosomal ribonucleoprotein autoantibodies in systemic lupus erythematosus / P. L. Meroni, G. de Bartolo, W. Barcellini, P. S. Riboldi, R. Basile, C. Betterle, C. Zanussi. - In: JOURNAL OF CLINICAL IMMUNOLOGY. - ISSN 0271-9142. - 4:1(1984 Jan), pp. 45-54. [10.1007/BF00915287]

Anti-ribosomal ribonucleoprotein autoantibodies in systemic lupus erythematosus

P.L. Meroni
Primo
;
P.S. Riboldi;
1984-01

Abstract

Sera from systemic lupus erythematosus (SLE) patients giving a fluorescent ribosomal pattern on tissue and cell preparations also showed precipitating autoantibodies against purified rat liver ribosomes. Ribosomal antigen is also present in rabbit thymus cellular extract (RTE), since the same sera gave precipitin lines against RTE in identity with ribosomes. Immunofluorescent staining was completely inhibited by serum absorption with ribosomes or with RTE. However ribosomal RNA and RNase or trypsin-treated ribosomes failed to react with these autoantibodies as demonstrated in immunoabsorption and immunodiffusion studies. These data suggest that these sera contain autoantibodies directed against some antigenic site composed of a portion of both RNA and ribosomal protein. Ribosomal autoantibodies were detectable at a low frequency in SLE patients characterized by an active disease and renal involvement.
Antigens; Lupus Erythematosus, Systemic; Autoantibodies; Immunodiffusion; Cells, Cultured; Humans; Ribonucleoproteins; Ribosomes; Fluorescent Antibody Technique
Settore MED/16 - Reumatologia
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/196521
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