19-methyl-[75Se]-selenocholesterol and 6-[75Se]-methyl-selenomethyl-19-nor-cholest-5(10)-en-3β-ol have been suggested as possible adrenal scanning agents. This study compares these selenium-labelled cholesterol analogues and cholesterol in some of the metabolic transformations in which cholesterol is involved in mammalian systems. The activity of cholesterol 7a-hydroxylase, lecithin: cholesterol acyltransferase, microsomal cholesterol acyltransferase and the sterol side-chain cleavage enzyme systems to transform the mentioned cholesterol analogues has been investigated. The uptake and secretion in bile of 19-methyl-[75Se]-selenocholesterol and 6-[75Se]-methyl-selenomethyl-19-nor-cholest-5(10)-en-3β-ol has been evaluated. The tissue distribution of these sterol analogues has been studied in rats. The findings suggest that 6-[75Se]-methyl-seleno-methyl-19-nor-cholest-5(10)en-3β-ol should be a useful agent for adrenal scanning.
|Titolo:||In vitro and in vivo studies on two 75Se labelled cholesterol analogues: 19-methyl-[75Se]-selenocholesterol and 6-[75Se]-methyl-selenomethyl-19-nor-cholest-5(10)-en-3 beta-ol|
BOSISIO, ENRICA ANGELA (Primo)
|Parole Chiave:||Rats; Cholesterol 7-alpha-Hydroxylase; Selenium; Animals; Cattle; Bile; Cholesterol Side-Chain Cleavage Enzyme; Radioisotopes; Sterol O-Acyltransferase; Cholesterol; Male; Female|
|Settore Scientifico Disciplinare:||Settore BIO/14 - Farmacologia|
|Data di pubblicazione:||ago-1979|
|Digital Object Identifier (DOI):||10.1016/0022-4731(79)90161-4|
|Appare nelle tipologie:||01 - Articolo su periodico|