19-methyl-[75Se]-selenocholesterol and 6-[75Se]-methyl-selenomethyl-19-nor-cholest-5(10)-en-3β-ol have been suggested as possible adrenal scanning agents. This study compares these selenium-labelled cholesterol analogues and cholesterol in some of the metabolic transformations in which cholesterol is involved in mammalian systems. The activity of cholesterol 7a-hydroxylase, lecithin: cholesterol acyltransferase, microsomal cholesterol acyltransferase and the sterol side-chain cleavage enzyme systems to transform the mentioned cholesterol analogues has been investigated. The uptake and secretion in bile of 19-methyl-[75Se]-selenocholesterol and 6-[75Se]-methyl-selenomethyl-19-nor-cholest-5(10)-en-3β-ol has been evaluated. The tissue distribution of these sterol analogues has been studied in rats. The findings suggest that 6-[75Se]-methyl-seleno-methyl-19-nor-cholest-5(10)en-3β-ol should be a useful agent for adrenal scanning.

In vitro and in vivo studies on two 75Se labelled cholesterol analogues: 19-methyl-[75Se]-selenocholesterol and 6-[75Se]-methyl-selenomethyl-19-nor-cholest-5(10)-en-3 beta-ol / E. Bosisio, G. S. Boyd. - In: JOURNAL OF STEROID BIOCHEMISTRY. - ISSN 0022-4731. - 11:2(1979 Aug), pp. 1113-1119.

In vitro and in vivo studies on two 75Se labelled cholesterol analogues: 19-methyl-[75Se]-selenocholesterol and 6-[75Se]-methyl-selenomethyl-19-nor-cholest-5(10)-en-3 beta-ol

E. Bosisio
Primo
;
1979

Abstract

19-methyl-[75Se]-selenocholesterol and 6-[75Se]-methyl-selenomethyl-19-nor-cholest-5(10)-en-3β-ol have been suggested as possible adrenal scanning agents. This study compares these selenium-labelled cholesterol analogues and cholesterol in some of the metabolic transformations in which cholesterol is involved in mammalian systems. The activity of cholesterol 7a-hydroxylase, lecithin: cholesterol acyltransferase, microsomal cholesterol acyltransferase and the sterol side-chain cleavage enzyme systems to transform the mentioned cholesterol analogues has been investigated. The uptake and secretion in bile of 19-methyl-[75Se]-selenocholesterol and 6-[75Se]-methyl-selenomethyl-19-nor-cholest-5(10)-en-3β-ol has been evaluated. The tissue distribution of these sterol analogues has been studied in rats. The findings suggest that 6-[75Se]-methyl-seleno-methyl-19-nor-cholest-5(10)en-3β-ol should be a useful agent for adrenal scanning.
Rats; Cholesterol 7-alpha-Hydroxylase; Selenium; Animals; Cattle; Bile; Cholesterol Side-Chain Cleavage Enzyme; Radioisotopes; Sterol O-Acyltransferase; Cholesterol; Male; Female
Settore BIO/14 - Farmacologia
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/196506
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